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    Sbus

    SampleBus

    Advanced Autosampling for AMBR® 250 Multi-Parallel Bioreactors

    Built on the back of Securecell’s own next-gen high-throughput Integra1 64 development, we have discovered a seamless automated flow to continuously sample each bioreactor of up to six AMBR® 250 systems four times every 24 hours.

    This highly customizable solution delivers the ultimate control together with Siemens PLC and Lucullus Bioprocess SCADA. This set-up works day and night with the industry’s top analyzers to achieve the highest level of insight for process development and optimization for your AMBR® 250 systems.

    Module Basic
    AMBR® 250 Super Sampling Process

     

    AMBR® 250 Super Sampling Process Flow

    1. Load 3 empty vials on conveyor belt
    2. Sampling process: transfer 1.5ml sample into one vial via Sample Transfer Unit
    3. Transfer sample vial and two empty vials Into High Density Filtration / Sample Prep Station
    4. Return sample vial with 0.5ml Cell Suspension to conveyor belt
    5. Transfer suspension sample to Cell Analytics (vortex, dilution)
    6. Transfer cell free sample (0.8ml) to cenveyor belt
    7. Deliver 100ul sample to UPLC Amino Acid Analyzer
    8. Deliver 100ul sample to 8 CQA Product Analyzer
    9. Deliver 500ul to Substrate Analyzer (glucose, lactate, NH4, ions)

    Expanded System Architecture

    Example: 4 AMBR® 250 Systems Connected by Bus and Sub-Bus to Analytics

    example-Sbus

     

    1. Vial Transfer Unit transfers vial from Sub-Bus to Main-Bus
    2. Sample Transfer Unit transfers processed sample into vial
    3. High-Density Filtration and Sample Prep Station (HDF-SPS) performs filtration up to 150 million cells per mL

    Filtration

    HDF-SPS (High Density Filtration and Sample Prep Station)

    Key Features:

    • Sampling via AMBR® 250 < 2ml into SEC cell (qualified in Numera project)
    • Centrifugation for cell and debris removal 2000g - 10,000g
    • Optional final Filtration via Sephara™ Membrane (yield 1.0 ml)
    • Any Cell Concentration up to 200 E6 Cells per ml can be filtrated with exceptional yield of supernatant
    • Screenshot 2024-06-17 095532
       
      1. Sample Sub-Bus
      2. Rotary gripper
      3. 2D-Code scanner
      4. Centrifuge
      5. Vortex mixer
      6. Scale
      7. Syringe 5 μl
      8. Syringe 1 μl
      9. Vial magazine at 4°C
      10. Vial magazine at 37°C
      11. Vial magazine at 80-90°C
      12. Vial Magazine at room temp.
      13. Counterweights
      14. Portal robot
      15. Waste bin

       

    PAT Performance Capabilities

    • sbus1

      It’s Time to Update Your Algorithms

      • Up to 6 Bioreactors from 6 AMBR® 250 systems can be sampled in parallel

      • Each bioreactor can be sampled every 6 hours or 4 X per day (8 X per day is possible with additional analytical equipment*)

      • Compatible with most analytical systems on the market (Cedex®, UPLC/LCMS, Nova FLEX, etc.)

    • sbus2

      Low Volume is Key

      • Sampling relies on Sephara™ Membrane technology

      • Highly reliable filtration behavior

      • 1200 Filtration cycles proved with CHO cell fermentation

      • Pore sizes: 0.4 - 32 micrometers

      • Quantifiable filtrate amount from 100 uL to 1 mL in steps of 10 uL

    • sbus3

      Structure of Vial Holder

      Each vial holder has several functional attributes that allow you to insert, transport, unload and remove vials from the structure.

      Along conveyor belt, several functional stations can be allocated to serve different functions including loading, unloading, and sample transfer.

       

    Sample Bus Concept

    Vial Loading Station

     

    • The loading station can host 16 racks with total 2,048 vials
    • Elevator mechanism removes exhausted rack from loading position
    • Station can run unsupervised, providing uninterupted sample vials for up to 5 days
    • High precision and transport security

    sbus4

     

    Usability Concept

    Local Interaction

     

    • Simple, intuitive, powerful control at your fingertips.
    • Siemens Panel
    • No Planning necessary. All managed by implicit rules
    • Lucullus-Interactions limited to administrator access

    sbus5

     

    Insights

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