Introduction
Gas supply in fermentation and cell culture processes carried out in bioreactor systems is highly important. In particular, in aerobic processes supplying a sufficient amount of oxygen is crucial for the performance of the culture. The rate at which oxygen is transferred from the gas phase to the liquid phase, the Oxygen Transfer Rate (OTR) should exceed the rate at which oxygen is consumed by the cells, the Oxygen Uptake Rate (OUR). Since many different factors influence gas transfer, each bioprocess is unique in this regard. Therefore, it is common practice to determine the efficiency at which oxygen is transferred for those particular process conditions. The volumetric gas transfer coefficient (kLa) is a direct indicator for this efficiency and can be experimentally determined using various methods. These methods tend to be labor-intensive and time-consuming. In this webinar, a novel Lucullus® application will be presented, which is capable of autonomously executing kLa determination experiments and calculating kLa values.
In this webinar, the following topics have been covered:
- Introduction to gas transfer in bioreactor systems
- Experimental determination of kLa
- Overview of the Lucullus® Operation for determining kLa
- Example of experimental results
- Q&A
Duration: 60 min
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